2012-11-08 10:15:35 - The kit first allows for the isolation of total DNA, including bacterial DNA, from the urine samples using a convenient spincolumn
Accuvis Bio, Abu Dhabi, UAE is now dealing with Norgen’s Urine-Based Malaria PCR Detection Kit constituents a complete, ready-to-use system for the isolation and detection of Malaria using end-point PCR. . The DNA is isolated free from PCR inhibitors, and can then be used as the template in a PCR reaction for Malaria detection using the provided Malaria Master Mix. The Malaria Master Mix contains reagents and enzymes for the specific amplification of a 350 bp region of the alaria DNA. In addition, Norgen’s Urine-Based Malaria PCR Detection Kit contains a second heterologous amplification system and controls to identify possible false negatives and/or inadequate isolation. The kit is designed to allow for the testing of 24 samples and is ideal
for use in surveillance of drug resistant pathogens, epidemiological studies, field surveillance of pathogens and surveys. The Malaria PCR Primer Set and Controls are also available separately for end-point PCR detection.
Malaria is a mosquito-borne infectious disease and considered one of the leading causes of death in the world. Malaria is a fatal tropical disease that is caused by a parasite knows as Plasmodium. It is usually spread through the bite of an infected female mosquito. It is estimated that there are 300-500 million new infected cases every year, with 1.5-2.7 million deaths worldwide. Most of the malaria-related deaths occur in sub-Saharan Africa. While blood is the most frequently used sample for diagnosis, both saliva and urine have been investigated as alternative, less invasive specimens.
Urine-Based Malaria PCR Detection Kit Benefits:
A complete optimized kit for isolation, detection and differentiation: The Urine-Based Malaria PCR Detection Kit is a complete kit that contains: 1) all the required components to allow for optimized isolation of viral DNA from urine, and 2) Malaria Master Mix and controls to allow for the optimized amplification and detection of the viral DNA.
Purchase Primer Set and Controls Separately: The Malaria Primer Set, and positive/negative controls can be
High Sensitivity and Specificity: The limit of detection is less than 860 copies/mL of urine.
Rapid Procedure: Isolate, amplify and detect Malaria in under 3 hours.
Includes controls to identify PCR inhibition and problems with DNA isolation: The Malaria 2X PCR Master Mix contains an independent PCR control to identify false negatives. A Malaria Isolation Control (IsoC) is also supplied to allow the user to control the DNA isolation procedure.
Non-invasive, non-infectious sample source: Urine is not infectious for most human diseases, making it an
attractive alternative for diagnostics.
Ideal for use in a number of different applications: Ideal for use in surveillance of drug resistant pathogens, epidemiological studies, field surveillance of pathogens and surveys.
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