The controversial embrace beat other famous clinches from romantic classics including 'Gone With The Wind' and 'Dirty Dancing' to top the online poll conducted by DVD rental firm LOVEFiLM.com.
Audrey Hepburn and George Peppard's romantic scene in 'Breakfast at Tiffany' s' was voted second, while Brad Pitt and Angelina Jolie's passionate kiss in 'Mr. and Mrs. Smith' came third.
Clark Gable and Vivien Leigh took fourth place with their tender moment in 'Gone With The Wind'.
Sarah Michelle Gellar and Selma Blair lesbian clinch in 'Cruel Intentions' claimed fifth position.
Other moments featured on the list include Colin Firth and Renee Zellweger's kiss in 'Bridget Jones' Diary' and Toby Maguire and Kirsten Dunst locking lips in 'Spiderman'.
Fliss White, marketing manager for LOVEFiLM, said: 'When we asked our members to share their favourite movie kiss, we never expected the results to be so varied. Our top 10 has covered everything from sexy passionate embraces to more sinister, threatening kisses.'

Top 10 best screen kisses of all time:

1. Jake Gyllenhaal and Heath Ledger in 'Brokeback Mountain'
2. Audrey Hepburn and George Peppard in 'Breakfast at Tiffany's'
3. Brad Pitt and Angelina Jolie in 'Mr. and Mrs. Smith'
4. Clark Gable and Vivien Leigh in 'Gone With The Wind'
5. Sarah Michelle Gellar and Selma Blair in 'Cruel Intentions'
6. Deborah Kerr and Burt Lancaster in 'From Here To Eternity'
7. Al Pacino and John Cazale in 'Godfather'
8. Colin Firth and Renee Zellweger in 'Bridget Jones' Diary'
9. Toby Maguire and Kirsten Dunst in 'Spiderman'
10. Patrick Swayze and Jennifer Gray in 'Dirty Dancing'