2008-10-29 10:51:08 -
www.researchandmarkets.com - Research and Markets (www.researchandmarkets.com/research/db3f79/gene_transfer_del) has announced the addition of the "Gene Transfer: Delivery and Expression of DNA and RNA, A Laboratory Manual" report to their offering.
Understanding gene function and regulation requires rigorous testing in live cells and organisms. Recent advances have provided a variety of new strategies for delivering DNA and RNA into cells and probing
their expression, as well as new clinical applications that rely upon the introduction of genetic material. The vast number of available techniques for clinical and laboratory research often makes selecting the optimal method a difficult process.
Gene Transfer: Delivery and Expression of DNA and RNA provides the first comprehensive guide to technical approaches for delivering nucleic acids into cells and organisms and of ensuring (even manipulating) appropriate expression. The detailed, step-by-step protocols cover a variety of methods, both well established and newly evolving. These include viral and nonviral methods of gene delivery, transgenic approaches, strategies for the regulation of transgene expression, and modification of the host response. The introductory matter to each chapter includes concise technical and theoretical discussions with considerations for selection of the appropriate system and strategies for delivery.
Key Topics Covered:
VIRAL VECTORS
- Retroviral Vectors
- Development of Lentiviral Vectors Expressing siRNA
- HIV-2 Vectors in Human Gene Therapy: Design, Construction, and Therapeutic Potential
- SIV Vectors as Vehicles for DNA Delivery
- Production and Use of Feline Immunodeficiency Virus-based Lentiviral Vectors
- Lentivirus Transduction of Hematopoietic Cells
- Spleen Necrosis Virus-based Vectors
- Foamy Virus Vector Production and Transduction of Hematopoietic Cells
- Simian Foamy Virus Type-1 Vectors
- Generation of VSV-G-pseudotyped Retroviral Vectors
- Targeted Gene Transfer with Surface-engineered Lentiviral Vectors
- Preparation of Pseudotyped Lentiviral Vectors Resistant to Inactivation by Serum Complement
- Generation of 2A Peptide-linked Multicistronic Vectors
- Construction of First-generation Adenoviral Vectors
- Production and Characterization of Helper-dependent Adenoviral Vectors
- Cell and Tissue Targeting
- Stable Producer Cell Lines for AAV Assembly
- Strategies for the Design of Hybrid Adeno-associated Virus Vectors
- Recombinant Herpes Simplex Virus Vectors
- Herpes Simplex Virus Type-1-derived Amplicon Vectors
- ?-2 Herpesvirus Saimiri-based Vectors
- Gene Delivery Using HSV/AAV Hybrid Amplicon Vectors
- Polyomaviruses: SV40
- SV40 In Vitro Packaging: A Pseudovirion Gene Delivery System
- Baculovirus-based Display and Gene Delivery Systems
- Safe, Simple, and High-capacity Gene Delivery into Insect and Vertebrate Cells by Recombinant Baculoviruses
- Alphaviruses: Semliki Forest Virus and Sindbis Virus as Gene Delivery Vectors
- Gene Transfer into Mammalian Cells Using Targeted Filamentous Bacteriophage
- Selection, Isolation, and Identification of Targeting Peptides for Ligand-directed Gene Delivery
- Rescue and Propagation of Tropism-modified Measles Viruses
- Picornavirus-based Expression Vectors
- Reverse Genetics of Influenza Viruses
NONVIRAL TECHNIQUES AND VECTORS
- An Overview of Condensing and Noncondensing Polymeric Systems for Gene Delivery
- Transfection of Hippocampal Neurons with Plasmid DNA Using Calcium Phosphate Coprecipitation
- Gene Delivery to Skin Using Biolistics
- Optimizing Electrotransfection of Mammalian Cells In Vitro
- Micro In Utero Electroporation for Efficient Gene Targeting in Mouse Embryos
- Lipoplex and LPD Nanoparticles for In Vivo Gene Delivery
- Bioresponsive Targeted Charge Neutral Lipid Vesicles for Systemic Gene Delivery
- HVJ Liposomes and HVJ Envelope Vectors
- Polylysine Copolymers for Gene Delivery
- PEI Nanoparticles for Targeted Gene Delivery
- Cyclodextrin-containing Polycations for Nucleic Acid Delivery
- Bionanocapsules Using the Hepatitis B Virus Envelope L Protein
- Formulations of Solid Lipid Nanoparticles for Transfection of Mammalian Cells In Vitro
- PEGylated Poly-l-lysine DNA Nanoparticles
- Water-soluble Lipopolymers and Lipopeptides for Nucleic Acid Delivery
- Cationic Polysaccharides for DNA Delivery
- Sustained Release of Plasmid DNAs Encoding Platelet-derived Growth Factor and Hyaluronan Synthase 2 from Cross-linked Hyaluronan Matrices and Films
- Linear Polyethylenimine: Synthesis and Transfection Procedures for In Vitro and In Vivo
- Protein Nanospheres for Gene Delivery: Preparation and In Vitro Transfection Studies with Gelatin Nanoparticles
- Vesicular Stomatitis Virus-G Conjugate
- High-throughput Methods for Screening Polymeric Transfection Reagents
- Poly(Lactic Acid) and Poly(Ethylene Oxide) Nanoparticles as Carriers for Gene Delivery
- Biodegradable Nanoparticles
- Transposon-mediated Delivery of Small Interfering RNA: Sleeping Beauty Transposon
- Efficient DNA Delivery into Mammalian Cells by Displaying the TAT Transduction Domain on Bacteriophage ? J. Wadia, A.
- Cell-penetrating Peptide-mediated Delivery of Peptide Nucleic Acid Oligomers
REGULATION OF TRANSGENE EXPRESSION
- Conditional Mutagenesis of the Genome Using Site-specific DNA Recombination
- Expression and Validation of Ribozyme and Short Hairpin RNA in Mammalian Cells
- Mifepristone-inducible Gene Regulatory System
- Dimerizer-mediated Regulation of Gene Expression
- RheoSwitch System: A Highly Sensitive Ecdysone Receptor-based Gene Regulation System Induced by Synthetic Small-molecule Ligands
- Site-specific Integration with Phage fC31 Integrase
- Creating Zinc Finger Nucleases to Manipulate the Genome in a Site-specific Manner Using a Modular-assembly Approach
SPECIALIZED TECHNIQUES OF GENE AND VECTOR DELIVERY
- Assembly of De Novo Bacterial Artificial Chromosome-based Human Artificial Chromosomes
- Delivery of Naked DNA Using Hydrodynamic Injection Techniques
- Nonviral Gene Transfer across the Blood-brain Barrier with Trojan Horse Liposomes
- Sonoporation: An Efficient Technique for the Introduction of Genes into Chick Embryos
- Genetic Manipulation of Mammalian Cells by Microinjection
- Magnetofection
- Photochemical Internalization for Light-directed Gene Delivery
TRANSGENIC APPROACHES
- Pronuclear Microinjection in Mice
- Knockdown Transgenic Mice Generated by Silencing Lentiviral Vectors
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