2013-09-22 12:22:26 - Dan Dongeun Huh, Wilf Family Term Chair & Assistant Professor of Bioengineering at the University of Pennsylvania, will speak about lung-on-a-chip 3D model systems at the 8th Cell Based Assay & Screening Technologies Conference on November 6-8, 2013 in San Francisco, CA.
One of the major problem slowing the development and regulatory approval of new and safer medical products is the lack of experimental in vitro model systems that can replace costly and time-consuming animal studies by predicting drug efficacy and toxicity in humans. Dr. Huh, whose research focuses on developing bioinspired microsystems that mimic the complex functionality of living human organs, will describe one such system that reconstitutes the critical functional alveolar-capillary interface of the human lung. This microdevice reproduces complex integrated organ-level responses to bacteria and inflammatory cytokines introduced into the alveolar space by inducing expression of intercellular adhesion molecule-1 (ICAM-1) on the microvascular endothelium surface, adhesion of circulating blood-borne neutrophils and their transmigration across the capillary-alveolar interface, and phagocytosis
of the infectious pathogens.
Using this approach, Dr. Huh and his lab developed novel nanotoxicology models and revealed that physiological cyclic mechanical strain greatly enhances toxic and inflammatory responses of the lung to silica nanoparticles and nanoparticle uptake by the epithelial cells, and stimulates their transport into the underlying microvasculature. The lab also observed similar effects of physiological breathing on nanoparticle absorption in the whole lung using a mouse lung ventilation-perfusion model.
In addition, the lab explored the potential use of this microsystem for the development of microengineered models of human lung disease for applications in drug screening. Given these results, this mechanically active biomimetic microsystem represents valuable new model systems for in vitro analysis of various physiological functions and disease processes, in addition to providing low-cost alternatives to animal and clinical studies for drug screening and toxicology applications.
For more information, please visit: www.gtcbio.com/assay.