2009-11-10 12:33:09 -

Scarab Genomics has made a series of planned, precise deletions to the E.

coli K-12 genome, reducing the genome by more than 15%. The multiple-deletion series (MDS™) strains eliminate non-essential genes, cryptic virulence genes, and sequences of recombinogenic and mobile insertion sequence (IS) DNA, while preserving robust growth in rich and minimal media. Removal of IS elements, which are a prevalent but frequently overlooked problem, improves clone stability. Prophage, which can be stress activated and cause premature lysis and failed fermentation runs, as seen with BL21(DE3), have been removed from these strains. Genome reduction optimizes Clean Genome E. coli for recombinant DNA and protein production, providing superior genomic and plasmid stability, increased cloning success with “unclonable” genes, and lower background

for downstream processing, … making them the strains of choice for manufacturing biotherapeutics.

About Scarab Genomics, LLC:


Scarab Genomics, a Madison, WI biotechnology company, offers unique Clean Genome ® E. coli Strains for next generation DNA and protein production. Based on Dr. Fred Blattner’s expertise in the new technology of genome engineering, the K-12 genome has been reduced to remove non-essential genes and problematic DNA elements. Genome reduction provides enhanced genetic stability and improved host safety, while retaining robust growth on minimal media, making these strains ideal for biotherapeutic production. Additional reduced strains are currently being produced and tested. Please inquire for additional information.



Scarab GenomicsTony Pharo, 888-513-7075 or 608-257-1624 www.ScarabGenomics.com :