2012-11-14 09:11:22 - AccuVis Bio, Abu Dhabi, UAE, is now dealing with Norgen’s Urine-Based XMRV RT-PCR Detection Kit constituents a complete, ready-to-use system for the isolation and detection of XMRV using end-point RT-PCR.
The kit first allows for the isolation of total RNA, including viral RNA, from the urine samples using a convenient spin-column. The RNA is isolated free from PCR inhibitors, and can then be used as the template in an RT-PCR reaction for XMRV detection using the provided XMRV Master Mix. The XMRV Master Mix contains reagents and enzymes for the specific amplification of a 300 bp region of XMRV. In addition, Norgen’s Urine-Based XMRV RT-PCR Detection Kit contains a second heterologous amplification system and controls to identify possible false negatives and/or inadequate isolation. The kit is designed to allow for the testing of 24 samples and is ideal for use in surveillance of drug resistant pathogens, epidemiologial studies, field surveillance
of pathogens and surveys. The XMRV PCR Primer Set and Controls are also available separately for end-point PCR detection.
Xenotropic murine leukemia virus-related virus (XMRV) belongs to the family Retroviridae and the genus gammaretrovirus. The virus was first described in 2006 and has since been isolated from human biological samples. XMRV has a single-stranded RNA genome that replicates through a DNA intermediate. The virus gets its name due to its close relationship with the murine leukemia viruses (MuLVs). The viral genome is approximately 8100 nucleotides in length and is 95% identical with several endogenous retroviruses of mice. While gammaretroviruses have well-characterized oncogenic effects in animals, they have not been shown to cause human cancers. However, XMRV was recently discovered in human prostate cancers and is the first gammaretrovirus known to infect humans. In addition to prostate cancer, a possible association with chronic fatigue syndrome has been reported, however it has yet to be established whether XMRV is a cause of this disease.
Urine-Based XMRV RT-PCR Detection Kit Benefits
A complete optimized kit for isolation, detection and differentiation: The Urine-Based XMRV RT-PCR Detection Kit is a complete kit that contains: 1) all the required components to allow for optimized isolation of viral RNA from urine, and 2) XMRV Master Mix and controls to allow for the optimized amplification and detection of the viral RNA.
Purchase Primer Set and Controls Separately: The XMRV Primer Set, and positive/negative controls can be
High Sensitivity and Specificity: The limit of detection is less than 2000 VP/mL of urine.
Rapid Procedure: Isolate, amplify and detect XMRV in under 3 hours.
Includes controls to identify PCR inhibition and problems with DNA isolation: The XMRV 2X RT-PCR Master Mix contains an independent PCR control to identify false negatives. An XMRV Isolation Control (IsoC) is also supplied to allow the user to control the RNA isolation procedure.
Non-invasive, non-infectious sample source: Urine is not infectious for most human diseases, making it an
attractive alternative for diagnostics.
Ideal for use in a number of different applications: Ideal for use in surveillance of drug resistant pathogens, epidemiological studies, field surveillance of pathogens and surveys.
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